Abstract
The transmission, survival, and virulence of Borrelia burgdorferi depend upon the spirochete’s ability to modulate its transcriptome as it cycles between its arthropod vector and reservoir host. This complex adaptive process is collectively referred to as “host-adaptation.” The paucibacillary nature of borrelial infections precludes the detailed analysis of host adaptation within infected mammalian tissues. To circumvent this limitation, we (J Clin Invest 101:2240–2250, 1998) developed a model system whereby spirochetes are cultivated within dialysis membrane chambers (DMCs) surgically implanted within the peritoneal cavity of a rat. Spirochetes within DMCs are exposed to many, if not all, of the environmental signals and physiological cues required for mammalian host adaptation but are protected from clearance by the host’s immune system.
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Acknowledgments
The authors would like to thank Ms. Anna Allard for her superb technical assistance and Dr. Justin Radolf for his continued support of this work. This work is supported by NIH Grants AI029735 and AI 126146 and an American Heart Association Grant-in-Aid award.
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Generation of mammalian host-adapted Borrelia burgdorferi by cultivation in peritoneal dialysis membrane chamber implantation in rats. Subheading 3.2, Preparation of DMCs (MP4 25016 kb)
Generation of mammalian host-adapted Borrelia burgdorferi by cultivation in peritoneal dialysis membrane chamber implantation in rats. Subheading 3.3, Peritoneal implant procedure (MP4 313609 kb)
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Caimano, M.J. (2018). Generation of Mammalian Host-Adapted Borrelia burgdorferi by Cultivation in Peritoneal Dialysis Membrane Chamber Implantation in Rats. In: Pal, U., Buyuktanir, O. (eds) Borrelia burgdorferi. Methods in Molecular Biology, vol 1690. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7383-5_3
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DOI: https://doi.org/10.1007/978-1-4939-7383-5_3
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