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Determination of di−/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Diamine and polyamine catabolism controls plant development, resistance to pathogens and stress responses. Diamine and polyamine oxidases control the catabolism of diamines and polyamines, respectively. Two major routes of di−/polyamine catabolism exist: the terminal and the interconverting. The in vitro activity of each route is assayed by the colorimetric or chemiluminescent determination of hydrogen peroxide produced by oxidation of di−/polyamine substrates. However, these assays fail to estimate activity of individual di−/polyamine oxidase isoenzymes. Herein, I describe an assay for the simultaneous in-gel determination of terminal and interconverting di−/polyamine oxidase isoenzyme activities.

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Authors and Affiliations

  1. Department of Plant Biology and Linnean Center of Plant Sciences, Swedish University of Agricultural Sciences, BioCentrum, Uppsala, Sweden

    Panagiotis N. Moschou

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  1. Panagiotis N. Moschou
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Correspondence to Panagiotis N. Moschou .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Moschou, P.N. (2018). Determination of di−/Polyamine Oxidase Activity in Plants by an In-Gel Spermidine Oxidation Assay. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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