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Quantitative Trait Loci for Root Growth Response to Cadaverine in Arabidopsis

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

Root growth architecture is a major determinant of agricultural productivity and plant fitness in natural ecosystems. Here we describe the methods used in a Quantitative Trait Loci (QTL) study that allowed the identification of ORGANIC CATION TRANSPORTER 1 (OCT1) as a determinant of root growth response to cadaverine treatment in Arabidopsis thaliana. This protocol screens natural accessions to characterize the variation in root growth response to the naturally occurring polyamine cadaverine, then uses recombination mapping to identify loci that are responsible for the variation existing between two accessions with contrasting phenotypes.

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Acknowledgments

We thank S.-H. Su, A. Jancewicz and A. Strohm for comments, advice, and/or research contribution to the material included in this manuscript. Our work was made possible by grants from the National Science Foundation (IOS-1121694), the National Aeronautics and Space Administration (NNX14AT23G), and the University of Wisconsin–Madison College of Agricultural and Life Sciences Hatch program.

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Authors and Affiliations

  1. Laboratory of Genetics, University of Wisconsin-Madison, 425G Henry Mall, Madison, WI, 53706, USA

    Nicole M. Gibbs & Patrick H. Masson

  2. Department of Biological Sciences, Wright State University, 3640 Colonel Glenn Hwy, Dayton, OH, 45435, USA

    Laura Vaughn Rouhana

Authors
  1. Nicole M. Gibbs
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  2. Laura Vaughn Rouhana
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  3. Patrick H. Masson
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Corresponding author

Correspondence to Patrick H. Masson .

Editor information

Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Gibbs, N.M., Rouhana, L.V., Masson, P.H. (2018). Quantitative Trait Loci for Root Growth Response to Cadaverine in Arabidopsis. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

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