Abstract
Circulating microRNAs are increasingly being used as noninvasive prognostic and predictive biomarkers for various types of cancer. We describe a method that uses real-time quantitative PCR (qPCR) for establishing a signature plasma microRNA profile that can distinguish patients with osteosarcoma from healthy control samples.
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References
Mirabello L, Troisi RJ, Savage SA (2009) Osteosarcoma incidence and survival rates from 1973 to 2004: data from the surveillance, epidemiology, and end results program. Cancer 115(7):1531–1543
Calin GA, Croce CM (2006) MicroRNA signatures in human cancers. Nat Rev Cancer 6(11):857–866
Cortez MA, Ivan C, Zhou P, Wu X, Ivan M, Calin GA (2010) microRNAs in cancer: from bench to bedside. Adv Cancer Res 108:113–157
Cortez MA, Bueso-Ramos C, Ferdin J, Lopez-Berestein G, Sood AK, Calin GA (2011 Aug) MicroRNAs in body fluids—the mix of hormones and biomarkers. Nat Rev Clin Oncol 8(8):467–477
Allen-Rhoades W, Kurenbekova L, Satterfield L, Parikh N, Fuja D, Shuck RL, Rainusso N, Trucco M, Barkauskas DA, Jo E, Ahern C, Hilsenbeck S, Donehower LA, Yustein JT (2015) Cross-species identification of a plasma microRNA signature for detection, therapeutic monitoring, and prognosis in osteosarcoma. Cancer Med 4(7):977–988
Acknowledgments
This work was funded through the generous support of a St. Baldrick’s Foundation Fellowship, the Amschwand Sarcoma Cancer Foundation’s Dr. Stephan Fadem Fellowship, and a Conquer Cancer Foundation of ASCO Young Investigator Award, supported by WWWW Foundation (Quad W). Research is also supported by the Chair’s Grant U10 CA98543 and Human Specimen Banking Grant U24 CA114766 of the Children’s Oncology Group from the National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. Additional support for research is provided by a grant from the WWWW (QuadW) Foundation, Inc. (www.QuadW.org) to the Children’s Oncology Group. Any opinions, findings, and conclusions expressed in this material are those of the authors and do not necessarily reflect those of the American Society of Clinical Oncology®, the Conquer Cancer Foundation, or the WWWW Foundation (Quad W).
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Allen-Rhoades, W., Yustein, J.T. (2018). Detection of Plasma MicroRNA Signature in Osteosarcoma Patients. In: Wu, W. (eds) MicroRNA and Cancer. Methods in Molecular Biology, vol 1699. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7435-1_9
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DOI: https://doi.org/10.1007/978-1-4939-7435-1_9
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-7435-1
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