Abstract
The development of experimental systems that allow in vivo antigen tracking as well as the study of B cell dynamics in real time and in situ, have transformed our understanding of the “how, when and where” B lymphocytes find antigen at secondary lymphoid organs in the last 10 years. Here, I described one of these experimental models, which uses highly fluorescent particulate antigen and B cell receptor (BCR)-transgenic B cells labeled with long-term fluorescent probes, combined with confocal and multiphoton microscopy.
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Acknowledgments
This work was supported by grants from the Spanish Ministry of Economy (BFU2013-48828-P) and from the Worldwide Cancer Research (WCR; grant reference number 15-1322).
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Carrasco, Y.R. (2018). In Vivo Tracking of Particulate Antigen Localization and Recognition by B Lymphocytes at Lymph Nodes. In: Liu, C. (eds) B Cell Receptor Signaling. Methods in Molecular Biology, vol 1707. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7474-0_11
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DOI: https://doi.org/10.1007/978-1-4939-7474-0_11
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