Abstract
The discontinuous polyacrylamide gel electrophoresis system devised by Laemmli (Nature 227:680–685, 1970) has not only been used in numerous laboratories but has also been modified in several ways since its birth. In our laboratories, we use a modified Laemmli SDS-PAGE system for following protein purification as well as for analysis of certain protein-protein interactions, mainly involving filametous actin.
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References
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
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Backman, L., Persson, K. (2018). The No-Nonsens SDS-PAGE. In: Singleton, T. (eds) Schizosaccharomyces pombe. Methods in Molecular Biology, vol 1721. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7546-4_8
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DOI: https://doi.org/10.1007/978-1-4939-7546-4_8
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