Abstract
Ligand–receptor interactions triggering signal transduction components of many sensory pathways, remain elusive due to paucity of high-throughput screening methods. Here we describe our use of small molecule microarrays comprising of small glycans, amino and organic acids, salts, and other known chemoeffectors, for screening of ligands specific to various sensory receptors, followed by surface plasmon resonance to verify the veracity and to determine the affinity constants of the interactions. This methodology allows for rapid and identification of the direct ligand binding between the sensory receptors and their specific ligands.
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Day, C.J., Korolik, V. (2018). Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance. In: Manson, M. (eds) Bacterial Chemosensing. Methods in Molecular Biology, vol 1729. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7577-8_24
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DOI: https://doi.org/10.1007/978-1-4939-7577-8_24
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7576-1
Online ISBN: 978-1-4939-7577-8
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