Abstract
Metal nanoparticles, more specifically gold nanoparticles (AuNPs), have become increasingly popular in research due to their optical, thermal, and electronic properties. It is these properties that have made them excellent for usage in life science areas such as medical, biological imaging, and drug delivery. AuNPs have been applied to multimodal imaging techniques to assist in fluorescence, MRI, and CT and in diagnostics through surface-enhanced Raman spectroscopy (SERS). These techniques all rely on the interaction of nanoparticles with electromagnetic stimulation. Here, we describe an experimental approach to investigate the combinatorial effects of gold nanoparticles with ultra-short electrical pulses. Specifically, we investigate cell membrane interaction with AuNPs to obtain information at the cellular level of possible changes in the uptake mechanism of the AuNPs. Our goal is to study the possibility of designing AuNPs for use in biomedical studies such as neuron stimulation and brain mapping.
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Acknowledgments
This research was supported in part by the Air Force Office of Scientific Research under grant numbers. FA9550-15-1-0109 and FA9550-15-1-0513. The authors would like to thank Dr. Jody Cantu for assistance with electroporation experiments and Dr. German Plascencia-Villa for assistance with electron microscopy of the cells.
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Franklin, S.K., Vincent, B., Tek, S., Nash, K.L. (2018). Influence of External Electrical Stimulation on Cellular Uptake of Gold Nanoparticles. In: Santamaria, F., Peralta, X. (eds) Use of Nanoparticles in Neuroscience. Neuromethods, vol 135. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7584-6_9
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