Abstract
Chlamydomonas reinhardtii, a single-celled green alga, is a powerful microbial experimental system for understanding gene function. As a consequence of a high-quality genome sequence, community-wide efforts for gene model refinement and annotation, resources for strain collections and robust molecular techniques, research with this organism has significantly expanded in the past few decades. In two companion chapters, we outline colorimetric and fluorescence-based methodologies for genetic reporter systems in Chlamydomonas, which can be used to investigate and delineate gene expression and regulatory mechanisms. Here, we describe protocols for arylsulfatase activity assays using ARS2, activity of which can be measured either quantitatively or qualitatively, and in low (individual sample) or high (96-well format) throughput.
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Acknowledgments
IKB and CEB-H are supported by the Office of Biological and Environmental Research of the United States Department of Energy. We are grateful to Kevin Keck for critical reading and helpful comments.
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Blaby, I.K., Blaby-Haas, C.E. (2018). Gene Expression Analysis by Arylsulfatase Assays in the Green Alga Chlamydomonas reinhardtii . In: Damoiseaux, R., Hasson, S. (eds) Reporter Gene Assays. Methods in Molecular Biology, vol 1755. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7724-6_11
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DOI: https://doi.org/10.1007/978-1-4939-7724-6_11
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