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High-Content Reporter Assays

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Reporter Gene Assays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1755))

Abstract

While luminescent reporter gene assays allow for a rapid and relatively interference free assessment of the activation state of a luminescent reporter, fluorescent reporters do not. They suffer from artifacts such as compound fluorescence and cellular debris which makes the assessment of whole well fluorescence signals difficult. However, the use of high-content screening allows for the isolation of individual cells, segmentation and thus enables the screener to utilize fluorescent reporters to assess the activation state of such a high-content reporter on a cell by cell level, thus minimizing artifacts. Here we discuss the use of such a high-content reporter that enables screening for compounds useful for HIV reactivation on Jurkat cells with high-content screening.

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Author information

Authors and Affiliations

  1. Screening & Protein Sciences, Merck Research Labs, Merck & Co., Inc., North Wales, PA, USA

    Jing Li & Matthew Tudor

  2. Lead Discovery and Optimization, Bristol Myers Squibb, Pennington, NJ, USA

    Erica Cook

  3. Screening and Translational Enzymology, Roche, Basel, Canton of Basel-Stadt, Switzerland

    Jeffrey Hermes

Authors
  1. Erica Cook
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  2. Jeffrey Hermes
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  3. Jing Li
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  4. Matthew Tudor
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Corresponding author

Correspondence to Matthew Tudor .

Editor information

Editors and Affiliations

  1. Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, California, USA

    Robert Damoiseaux

  2. Pfizer, Inc., Cambridge, Massachusetts, USA

    Samuel Hasson

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© 2018 Springer Science+Business Media, LLC, part of Springer Nature

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Cook, E., Hermes, J., Li, J., Tudor, M. (2018). High-Content Reporter Assays. In: Damoiseaux, R., Hasson, S. (eds) Reporter Gene Assays. Methods in Molecular Biology, vol 1755. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7724-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7724-6_13

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7722-2

  • Online ISBN: 978-1-4939-7724-6

  • eBook Packages: Springer Protocols

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