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Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing

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Cell-Based Microarrays

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1771))

Abstract

Biomolecule microarrays are generally produced by conventional microarrayer, i.e., by contact or inkjet printing. Microcontact printing represents an alternative way of deposition of biomolecules on solid supports but even if various biomolecules have been successfully microcontact printed, the production of biomolecule microarrays in routine by microcontact printing remains a challenging task and needs an effective, fast, robust, and low-cost automation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.

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Acknowledgments

This work was supported by the national research agency “LABCOM” program (ANR-13-LAB2-0009-01) and partly supported by LAAS CNRS micro and nanotechnology facilities platform (member of the French RENATECH network). We thank Charline Blatché for her assistance in the cell microarray fabrication.

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Authors and Affiliations

  1. Laboratory for Analysis and Architecture of Systems (LAAS-CNRS), Université de Toulouse, CNRS, INSA, Toulouse, France

    Julie Foncy, Aurore Estève, Camille Colin, Laurent Malaquin, Christophe Vieu & Emmanuelle Trévisiol

  2. INNOPSYS, Carbonne, France

    Amélie Degache & Jean Christophe Cau

Authors
  1. Julie Foncy
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  2. Aurore Estève
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  3. Amélie Degache
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  4. Camille Colin
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  5. Jean Christophe Cau
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  6. Laurent Malaquin
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  7. Christophe Vieu
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  8. Emmanuelle Trévisiol
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Corresponding author

Correspondence to Emmanuelle Trévisiol .

Editor information

Editors and Affiliations

  1. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Peter Ertl

  2. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Mario Rothbauer

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Foncy, J. et al. (2018). Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing. In: Ertl, P., Rothbauer, M. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 1771. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7792-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-7792-5_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7791-8

  • Online ISBN: 978-1-4939-7792-5

  • eBook Packages: Springer Protocols

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