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Dissection and Preparation of Human Primary Fetal Ganglionic Eminence Tissue for Research and Clinical Applications

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Huntington’s Disease

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1780))

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Abstract

Here, we describe detailed dissection and enzymatic dissociation protocols for the ganglionic eminences from the developing human brain to generate viable quasi-single cell suspensions for subsequent use in transplantation or cell culture. These reliable and reproducible protocols can provide tissue for use in the study of the developing human brain, as well as for the preparation of donor cells for transplantation in Huntington’s disease (HD). For use in the clinic as a therapy for HD, the translation of these protocols from the research laboratory to the GMP suite is described, including modification to reagents used and appropriate monitoring and tissue release criteria.

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Acknowledgments

The protocols described in this chapter were developed using fetal samples from the South Wales Initiative for Fetal Tissue (SWIFT) Research Tissue Bank, and the Cardiff Fetal Tissue Bank (CFTB). These tissue banks have received funding from the Medical Research Council, the Welsh Government, the Brain Repair and Intracranial Neurotherapeutics (BRAIN) Unit funded through Health and Care Research Wales, and EU FP7 projects TransEuro and Repair-HD. The authors would like to acknowledge the help and contributions of all past and present members of the SWIFT team from within the Brain Repair Group, Cardiff University, and University Hospital of Wales.

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Authors and Affiliations

  1. Brain Repair Group, School of Biosciences, Cardiff University, Cardiff, UK

    Victoria H. Roberton & Anne-Marie McGorrian

  2. Brain Repair Group, Cardiff University, Cardiff, UK

    Anne E. Rosser & Sophie V. Precious

Authors
  1. Victoria H. Roberton
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  2. Anne E. Rosser
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  3. Anne-Marie McGorrian
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  4. Sophie V. Precious
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Corresponding author

Correspondence to Sophie V. Precious .

Editor information

Editors and Affiliations

  1. Brain Repair Group, Cardiff University, Cardiff, United Kingdom

    Sophie V. Precious

  2. Brain Repair Group, Cardiff University, Cardiff, United Kingdom

    Anne E. Rosser

  3. Brain Repair Group, Cardiff University, Cardiff, United Kingdom

    Stephen B. Dunnett

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Roberton, V.H., Rosser, A.E., McGorrian, AM., Precious, S.V. (2018). Dissection and Preparation of Human Primary Fetal Ganglionic Eminence Tissue for Research and Clinical Applications. In: Precious, S., Rosser, A., Dunnett, S. (eds) Huntington’s Disease. Methods in Molecular Biology, vol 1780. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7825-0_26

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  • DOI: https://doi.org/10.1007/978-1-4939-7825-0_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7824-3

  • Online ISBN: 978-1-4939-7825-0

  • eBook Packages: Springer Protocols

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