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On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morphine Addiction

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Receptor-Receptor Interactions in the Central Nervous System

Part of the book series: Neuromethods ((NM,volume 140))

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Abstract

Receptor–receptor interactions that occur in G protein-coupled receptors (GPCRs) oligomers can be explored using three different functional approaches as starting point: (1) quantitative receptor autoradiography (saturation assay); (2) agonist-stimulated [35S]GTPγS binding in autoradiography; and (3) immunohistochemistry. Together, they allow to explore functional changes in receptors signaling transduction, i.e., receptor recognition, G protein activation, and downstream signaling cascades. Here, we describe these three selected methods that have been successfully employed in the study of the functional interaction of dopamine D4 (D4R) and μ opioid (MOR) receptors in the rat caudate putamen in the context of morphine addiction.

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Acknowledgments

This work has been supported by CTS161 (Junta de Andalucía, Spain).

Author information

Authors and Affiliations

  1. Facultad de Ciencias, Instituto de Investigación Biomédica, Universidad de Málaga, Málaga, Spain

    Alicia Rivera, Alejandra Valderrama-Carvajal, Kirill Shumilov & M. Ángeles Real

  2. Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, Université Libre de Bruxelles, Brussels, Belgium

    Diana Suárez-Boomgaard

  3. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Kjell Fuxe

  4. Facultad de Medicina, Instituto de Investigación Biomédica, Universidad de Málaga, Málaga, Spain

    Belén Gago

Authors
  1. Alicia Rivera
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  2. Alejandra Valderrama-Carvajal
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  3. Diana Suárez-Boomgaard
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  4. Kirill Shumilov
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  5. M. Ángeles Real
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  6. Kjell Fuxe
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  7. Belén Gago
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Corresponding author

Correspondence to Alicia Rivera .

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Editors and Affiliations

  1. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    KJELL FUXE

  2. Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden

    Dasiel O. Borroto-Escuela

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Rivera, A. et al. (2018). On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morphine Addiction. In: FUXE, K., Borroto-Escuela, D. (eds) Receptor-Receptor Interactions in the Central Nervous System. Neuromethods, vol 140. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8576-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8576-0_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8575-3

  • Online ISBN: 978-1-4939-8576-0

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