Abstract
The advent of high-speed atomic force microscopy (HS-AFM) over the recent years has opened up new horizons for the study of structure, function and dynamics of biological molecules. HS-AFM is capable of 1000 times faster imaging than conventional AFM. This circumstance uniquely enables the observation of the dynamics of all the molecules present in the imaging area. Over the last 10 years, the HS-AFM has gone from a prototype-state technology that only a few labs in the world had access to (including ours) to an established commercialized technology that is present in tens of labs around the world. In this protocol chapter we share with the readers our practical know-how on high resolution HS-AFM imaging.
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Acknowledgments
This work was supported by French National Institute of Health and Medical Research (Inserm); the program “Investissements d’Avenir” by the French National Research Agency, ANR-10-LABX-0083 (Labex EFL); Grant ANR-16-CE15-0023 (ANR SalmoTubes); Grant ANR-15-CE11-0020 (ANR MoBaRhE). A.M. acknowledges support from the Long Term EMBO Fellowship (ALTF 1427-2014) and the Marie Curie Action (MSCA-IF-2014-EF-655157).
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Zuttion, F., Redondo-Morata, L., Marchesi, A., Casuso, I. (2018). High-Resolution and High-Speed Atomic Force Microscope Imaging. In: Lyubchenko, Y. (eds) Nanoscale Imaging. Methods in Molecular Biology, vol 1814. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8591-3_11
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DOI: https://doi.org/10.1007/978-1-4939-8591-3_11
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