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Identification of microRNA Precursor-Associated Proteins

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miRNA Biogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1823))

Abstract

MicroRNA (miRNA) biogenesis is regulated intricately at multiple levels. In addition to transcriptional control of pri-miRNA loci, sequence as well as structural features of the pri-miRNA-stem loop determine its processing efficiency by the endonucleases Drosha and Dicer. On the one hand, general features are necessary to allow a hairpin to be recognized by the processing machinery; on the other hand, specific sequence motifs of individual miRNA precursors can be read by RNA binding proteins (RBPs) that regulate processing, leading to increased or decreased levels of functional miRNAs. In a pulldown experiment using the pri-miRNA hairpin as immobilized bait, cognate RBPs can be isolated and analyzed by immunoblotting or mass spectrometry, allowing for the discovery or analysis of protein regulators of miRNA biogenesis.

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Acknowledgements

Our research is supported by grants from the Deutsche Forschungsgemeinschaft (SFB 960, FOR2127, PP 1935, PP 1784) and the Bavarian Ministry for Education and Science (BioSysNet).

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Authors and Affiliations

  1. Laboratory for RNA Biology, Biochemistry Center Regensburg (BZR), University of Regensburg, Regensburg, Germany

    Thomas Treiber, Nora Treiber & Gunter Meister

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  1. Thomas Treiber
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  2. Nora Treiber
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  3. Gunter Meister
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Corresponding author

Correspondence to Gunter Meister .

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Editors and Affiliations

  1. Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark

    Ulf Andersson Vang Ørom

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Treiber, T., Treiber, N., Meister, G. (2018). Identification of microRNA Precursor-Associated Proteins. In: Ørom, U. (eds) miRNA Biogenesis. Methods in Molecular Biology, vol 1823. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-8624-8_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8624-8_9

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4939-8623-1

  • Online ISBN: 978-1-4939-8624-8

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