Abstract
Lymph collected from throughout the body is exclusively returned to blood circulation via two pairs of bilaterally located lymphovenous valves. Lymphovenous valves share numerous similarities with lymphatic and venous valves and are defective in multiple mouse models of lymphedema or lymphatic dysfunction. Here we describe a protocol that combines the strengths of fluorescence microscopy and scanning electron microscopy to precisely locate and analyze the topography of developing lymphovenous valves at high resolution.
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Acknowledgments
This work is supported by NIH/NHLBI (R01HL131652 and R01HL133216 to RSS), Oklahoma Center for Adult Stem Cell Research (4340) to RSS, NIH/NIGMS COBRE (P20 GM103441 to XG; PI: Dr. Rodger McEver) and American Heart Association (15BGIA25710032 for RSS).
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Geng, X., Srinivasan, R.S. (2018). Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development. In: Oliver, G., Kahn, M. (eds) Lymphangiogenesis. Methods in Molecular Biology, vol 1846. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8712-2_6
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DOI: https://doi.org/10.1007/978-1-4939-8712-2_6
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