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Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants

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Metabolic Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1862))

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Abstract

Bone is a metabolically dynamic tissue that is continuously built up and broken down through anabolic and catabolic processes regulated by a variety of systemic and local signaling molecules. Here, we describe quantitative multiplex immunoassay analysis of supernatants collected from cultured human bone tissue fragments to profile local factors associated with the bone turnover process.

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Acknowledgments

This work was supported by a grant from the METAvivor Foundation to BLK.

Author information

Authors and Affiliations

  1. MilliporeSigma Corporation, St Louis, MO, USA

    Wen-Rong Lie

  2. Department of Orthopaedic Surgery, Stanford University School of Medicine, Redwood City, CA, USA

    Derek F. Amanatullah

  3. Department of Pediatrics, Stanford University School of Medicine, Stanford, CA, USA

    Bonnie L. King

Authors
  1. Wen-Rong Lie
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  2. Derek F. Amanatullah
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  3. Bonnie L. King
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Editor information

Editors and Affiliations

  1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium

    Sarah-Maria Fendt

  2. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA

    Sophia Y. Lunt

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Lie, WR., Amanatullah, D.F., King, B.L. (2019). Quantitative Multiplex Immunoassay for Profiling Bone Turnover Biomarkers in Human Bone Tissue Culture Supernatants. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8769-6_18

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8768-9

  • Online ISBN: 978-1-4939-8769-6

  • eBook Packages: Springer Protocols

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