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Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration

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Hepatic Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1905))

Abstract

Following massive hepatocyte ablation in zebrafish, biliary epithelial cells can extensively give rise to hepatocytes through liver progenitor cells (LPCs). The zebrafish liver injury model is an important system to elucidate the molecular mechanisms underlying LPC-driven liver regeneration. Here, we describe a chemical screening method using the zebrafish model for identifying small molecules that can modulate LPC-driven liver regeneration.

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Acknowledgments

We thank Jackie Russell, Mehwish Khaliq, and Michael Tsang for editing the manuscript. This study was supported by an NIH grant (DK101426) to D.S.

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Authors and Affiliations

  1. Department of Developmental Biology, McGowan Institute for Regenerative Medicine, Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, USA

    Sungjin Ko & Donghun Shin

Authors
  1. Sungjin Ko
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  2. Donghun Shin
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Corresponding author

Correspondence to Donghun Shin .

Editor information

Editors and Affiliations

  1. Sapporo Medical University, Sapporo, Hokkaido, Japan

    Naoki Tanimizu

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Ko, S., Shin, D. (2019). Chemical Screening Using a Zebrafish Model for Liver Progenitor Cell-Driven Liver Regeneration. In: Tanimizu, N. (eds) Hepatic Stem Cells . Methods in Molecular Biology, vol 1905. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8961-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8961-4_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8960-7

  • Online ISBN: 978-1-4939-8961-4

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