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Physiological Characterization of Phototropism in Arabidopsis Seedlings

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Phototropism

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1924))

Abstract

To date, many mutants have been isolated from dicot plants, including Arabidopsis thaliana, and the physiological roles of the isolated genes have been identified. Molecular genetic analyses have usually been conducted in the model plant Arabidopsis to identify blue-light photoreceptors and key signaling components in phototropic responses. Despite these investigations, several molecular mechanisms involved in phototropism remain unknown, possibly because detailed physiological analyses have not been conducted properly in the isolated mutants. This chapter describes an approach for the detailed investigation of hypocotyl and root phototropism in Arabidopsis seedlings. The information provided here is expected to facilitate the analysis of phototropic responses in other plant species.

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Acknowledgments

We thank Professor Moritoshi Iino (Osaka City University) and the members of his laboratory for providing useful technical advice, and Professor Tatsuya Sakai (Niigata University) for providing useful comments and technical support. This work was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI (No. 24657027 and No. 17K07451 to K. H., No. 16J01942 to T. K.) and (in part) by a Grant for Basic Science Research Projects from the Sumitomo Foundation to K. H.

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Authors and Affiliations

  1. Department of Applied Chemistry, Faculty of Fundamental Engineering, Nippon Institute of Technology, Miyashiro, Saitama, Japan

    Ken Haga

  2. Graduate School of Science and Technology, Niigata University, Niigata, Japan

    Taro Kimura

Authors
  1. Ken Haga
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  2. Taro Kimura
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Corresponding author

Correspondence to Ken Haga .

Editor information

Editors and Affiliations

  1. Department of Biological Sciences, Faculty of Science, Hokkaido University, Sapporo, Japan

    Kotaro T. Yamamoto

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Haga, K., Kimura, T. (2019). Physiological Characterization of Phototropism in Arabidopsis Seedlings. In: Yamamoto, K. (eds) Phototropism. Methods in Molecular Biology, vol 1924. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9015-3_1

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  • DOI: https://doi.org/10.1007/978-1-4939-9015-3_1

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9014-6

  • Online ISBN: 978-1-4939-9015-3

  • eBook Packages: Springer Protocols

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