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Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling

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Calcium Signalling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1925))

Abstract

Calcium ion (Ca2+) is a ubiquitous intracellular messenger able to generate versatile intracellular signals that modulate a large variety of functions in virtually every cell type. Chemical and genetic biosensors, targeted to different subcellular compartments, have been developed and continuously improved to monitor Ca2+ dynamics in living cells. Here we describe the usage of Förster resonance energy transfer (FRET)-based Cameleon probes to investigate Ca2+ influx across the plasma membrane (PM) or Ca2+ release from the main intracellular Ca2+ store, the endoplasmic reticulum (ER).

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Acknowledgment

This work was supported by Telethon GGP16029, the University of Padua, and the EU Joint Programme for Neurodegenerative Disease Research (JPND) CeBioND, supported by the MIUR (DM 9; 08/01/2015).

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Authors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Luisa Galla, Paola Pizzo & Elisa Greotti

  2. Neuroscience Institute —Italian National Research Council (CNR), Padua, Italy

    Luisa Galla, Paola Pizzo & Elisa Greotti

Authors
  1. Luisa Galla
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  2. Paola Pizzo
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  3. Elisa Greotti
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Corresponding author

Correspondence to Elisa Greotti .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Anna Raffaello

  2. Department of Biomedical Sciences, University of Padua, Padua, Italy

    Denis Vecellio Reane

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Galla, L., Pizzo, P., Greotti, E. (2019). Exploiting Cameleon Probes to Investigate Organelles Ca2+ Handling. In: Raffaello, A., Vecellio Reane, D. (eds) Calcium Signalling. Methods in Molecular Biology, vol 1925. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9018-4_2

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  • DOI: https://doi.org/10.1007/978-1-4939-9018-4_2

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9017-7

  • Online ISBN: 978-1-4939-9018-4

  • eBook Packages: Springer Protocols

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