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Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE)

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Glutamate Receptors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1941))

Abstract

Probing of glutamate receptors at sub-millisecond time scale is a key element needed for understanding of their response kinetics, neural signal transduction, and, in a wider context, intercellular cross talk. One of the classical techniques used to obtain this type of data in electrophysiology is placing a recording pipette in front of a double-barrel solution application pipette, which provides a rapid switch between two solutions. Here we describe a modification of this classical technique, which utilizes a solution application pipette with several loading capillaries. Such a system is capable of replacing multiple application solutions within 7–12 s time intervals. This modified protocol enables ultrafast application of several solutions at the same set of receptors, thus allowing powerful paired-sample statistical approaches. In addition, the same experimental equipment fabricated for the ultra-resolution probing of receptor kinetics can also be applied in several other types of electrophysiological experiments.

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Author information

Authors and Affiliations

  1. CCBS, University of Edinburgh, Edinburgh, UK

    Nathanael O’Neill & Sergiy Sylantyev

Authors
  1. Nathanael O’Neill
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  2. Sergiy Sylantyev
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Corresponding author

Correspondence to Sergiy Sylantyev .

Editor information

Editors and Affiliations

  1. Department of Neurology, University of Wisconsin, Madison, WI, USA

    Corinna Burger

  2. ANSER, Homeland Security Studies and Analysis Institute, Falls Church, VA, USA

    Margaret Jo Velardo

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O’Neill, N., Sylantyev, S. (2019). Glutamate Receptor Probing with Rapid Application and Solution Exchange (RASE). In: Burger, C., Velardo, M. (eds) Glutamate Receptors. Methods in Molecular Biology, vol 1941. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9077-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-9077-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9076-4

  • Online ISBN: 978-1-4939-9077-1

  • eBook Packages: Springer Protocols

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