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RecET-Mediated Recombineering in Acinetobacter baumannii

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Acinetobacter baumannii

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1946))

Abstract

Acinetobacter baumannii rapidly acquires antibiotic resistance, and its genome encodes mechanisms to tolerate biocides and desiccation, enhancing its persistence in hospital settings. Tools to rapidly dissect the A. baumannii genome are needed to understand cellular factors that contribute to its resiliency at a genetic and mechanistic level. While a substantial amount of clinical data has documented the global rise of A. baumannii as an antibiotic-resistant pathogen, genetic tools to dissect its molecular details have been limited. This procedure describes a recombination-mediated genetic engineering (recombineering) system for targeted genome editing of A. baumannii. This system can perform directed mutagenesis on wide-ranging genes and operons and has broad application in various strains of A. baumannii.

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Acknowledgment

This work was supported by NIH grant AI125337 to B.W.D, NIH F32 GM116523 to A.T.T., NIH grants AI064184, AI76322, AI119879 and AI129940 to M.S.T., and NSF 2017238867 M. J. P.

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Authors and Affiliations

  1. Department of Molecular Biosciences, University of Texas at Austin, Austin, TX, USA

    Ashley T. Tucker & Bryan W. Davies

  2. Department of Infectious Diseases, University of Georgia, College of Veterinary Medicine, Athens, GA, USA

    Matthew J. Powers & M. Stephen Trent

  3. Department of Microbiology, University of Georgia, Athens, GA, USA

    Matthew J. Powers

  4. Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

  5. Center for Systems and Synthetic Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

  6. John Ring LaMontagne Center for Infectious Diseases, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, TX, USA

    Bryan W. Davies

Authors
  1. Ashley T. Tucker
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  2. Matthew J. Powers
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  3. M. Stephen Trent
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  4. Bryan W. Davies
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Corresponding author

Correspondence to Bryan W. Davies .

Editor information

Editors and Affiliations

  1. Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, Kansas City, KS, USA

    Indranil Biswas

  2. Department of Microbiology and Immunology, Emory University, Atlanta, GA, USA

    Philip N. Rather

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© 2019 Springer Science+Business Media, LLC, part of Springer Nature

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Tucker, A.T., Powers, M.J., Trent, M.S., Davies, B.W. (2019). RecET-Mediated Recombineering in Acinetobacter baumannii. In: Biswas, I., Rather, P. (eds) Acinetobacter baumannii. Methods in Molecular Biology, vol 1946. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9118-1_11

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  • DOI: https://doi.org/10.1007/978-1-4939-9118-1_11

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9117-4

  • Online ISBN: 978-1-4939-9118-1

  • eBook Packages: Springer Protocols

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