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Sepsis Induced by Cecal Ligation and Puncture

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Mouse Models of Innate Immunity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1960))

Abstract

Despite advances in intensive care unit interventions, including the use of specific antibiotics and anti-inflammation treatment, sepsis with concomitant multiple organ failure is the most common cause of death in many acute care units. In order to understand the mechanisms of clinical sepsis and develop effective therapeutic modalities, there is a need to use effective experimental models that faithfully replicate what occurs in patients with sepsis. Several models are commonly used to study sepsis, including intravenous endotoxin challenge, injection of live organisms into the peritoneal cavity, establishing abscesses in the extremities, and the induction of experimental polymicrobial peritonitis via cecal ligation and puncture (CLP). Here, we describe the surgical procedure of CLP in mice, which has been demonstrated to closely replicate the nature and course of clinical sepsis in human subjects.

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Acknowledgments

Haitao Wen is supported by the NIH grant R01GM120496.

Author information

Authors and Affiliations

  1. Department of Hepatobiliary Surgery and Liver Transplantation, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China

    Wei Gong

  2. Department of Microbial Infection and Immunity, The Ohio State University College of Medicine, Columbus, OH, USA

    Haitao Wen

  3. Infectious Disease Institute, The Ohio State University College of Medicine, Columbus, OH, USA

    Haitao Wen

Authors
  1. Wei Gong
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  2. Haitao Wen
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Corresponding author

Correspondence to Haitao Wen .

Editor information

Editors and Affiliations

  1. Department of Biomedical Sciences, Virginia-Maryland Regional College, Blacksburg, VA, USA

    Irving C. Allen

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Gong, W., Wen, H. (2019). Sepsis Induced by Cecal Ligation and Puncture. In: Allen, I. (eds) Mouse Models of Innate Immunity. Methods in Molecular Biology, vol 1960. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-9167-9_22

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  • DOI: https://doi.org/10.1007/978-1-4939-9167-9_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-9166-2

  • Online ISBN: 978-1-4939-9167-9

  • eBook Packages: Springer Protocols

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