Skip to main content
SpringerLink
Log in

An Overview of CE in Clinical Analysis

  • Protocol
  • First Online:
Clinical Applications of Capillary Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1972))

Abstract

The development and general applications of capillary electrophoresis (CE) in the field of clinical chemistry are discussed. It is shown how the early development of electrophoresis was closely linked to clinical testing. The rise of gel electrophoresis in clinical chemistry is described, as well as the eventual developments that lead to the creation and the use of modern CE. The general principles of CE are reviewed and the potential advantages of this method in clinical testing are examined. Finally, an overview is presented of several areas in which CE has been developed and is currently being explored for use with clinical samples.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Rifai N, Horvath AR, Wittwer CT (eds) (2018) Tietz textbook of clinical chemistry and molecular diagnostics, 6th edn. Amsterdam, Elsevier

    Google Scholar 

  2. Gornall AG (1986) Applied biochemistry of clinical disorders. Lippincott, New York

    Google Scholar 

  3. Hage DS (2016) Chromatography and electrophoresis. In: Clarke W (ed) Contemporary practice in clinical chemistry, 3rd edn. AACC Press, Washington, DC

    Google Scholar 

  4. Allen RC, Griffiths JC (1991) Electrophoresis. Anal Chem 63:209R–213R

    Google Scholar 

  5. Jorgenson JW (1986) Electrophoresis. Anal Chem 58:743A–760A

    Article  CAS  Google Scholar 

  6. Skoog DA, Holler FJ, Crouch SR (2017) Principles of instrumental analysis, 7th edn. Cengage Learning, Boston

    Google Scholar 

  7. Hage DS, Carr JD (2011) Analytical chemistry and quantitative analysis. Prentice Hall, Boston

    Google Scholar 

  8. Cazes J (ed) (2005) Ewing’s analytical instrumentation handbook, 3rd edn. CRC Press, Boca Raton

    Google Scholar 

  9. Karger BL, Snyder LR, Hovath C (1973) An introduction to separation science. Wiley, New York

    Google Scholar 

  10. Hardy WB (1899) On the coagulation of proteid by electricity. J Physiol 26:288–304

    Article  Google Scholar 

  11. Hardy WB (1905) Colloidal solution. The globulins. J Physiol 33:251–337

    Article  CAS  Google Scholar 

  12. Michaelis L (1909) Elektrische uberfuhrung von fermenten. Biochem Z 16:81–86

    CAS  Google Scholar 

  13. Tiselius AWK (1937) A new apparatus for electrophoretic analysis of colloidal mixtures. Trans Faraday Soc 33:524–531

    Article  CAS  Google Scholar 

  14. Wehr T, Zhu M (1994) Capillary electrophoresis: historical perspectives. In: Landers JP (ed) Handbook of capillary electrophoresis. CRC Press, Boca Raton

    Google Scholar 

  15. Hjerten S (1967) Free zone electrophoresis. Chromatogr Rev 9:122–219

    Article  CAS  Google Scholar 

  16. Virtanen R (1974) Zone electrophoresis in a narrow-bore tube employing potentiometric detection. Acta Polytech Scand Chem 123:1–67

    Google Scholar 

  17. Jorgenson JW, Lukacs KD (1981) Zone electrophoresis in open-tubular glass capillaries. Anal Chem 53:1298–1302

    Article  CAS  Google Scholar 

  18. Jorgenson JW, Lukacs KD (1981) High resolution separations based on electrophoresis and electroosmosis. J Chromatogr 218:209–216

    Article  CAS  Google Scholar 

  19. Jorgenson JW, Lukacs KD (1983) Capillary zone electrophoresis. Science 222:266–272

    Article  CAS  Google Scholar 

  20. Xu Y (1995) Capillary electrophoresis. Anal Chem 65:425R–433R

    Article  Google Scholar 

  21. Xu Y (1995) Capillary electrophoresis. Anal Chem 67:463R–473R

    Article  CAS  Google Scholar 

  22. Landers JP (1995) Clinical capillary electrophoresis. Clin Chem 41:495–509

    Article  CAS  Google Scholar 

  23. Petersen JR, Okorodudu AO, Mohammad A, Payne DA (2003) Capillary electrophoresis and its application in the clinical laboratory. Clin Chim Acta 330:1–30

    Article  CAS  Google Scholar 

  24. Phillips TM, Kalish H (eds) (2013) Clinical applications of capillary electrophoresis. Human Press, Totowa, NJ

    Google Scholar 

  25. Bojarski J, Szymura-Oleksiak J (2003) Applications of capillary electrophoresis in clinical analysis of drugs. In: Aboul-Enein HY (ed) Separation techniques in clinical chemistry. Marcel Dekker, New York

    Google Scholar 

  26. Moser AC, Willicott CW, Hage DS (2014) Clinical applications of capillary electrophoresis-based immunoassays. Electrophoresis 35:937–955

    Article  CAS  Google Scholar 

  27. Şahin A, Laleli YR, Ortancil R (1995) Haemoglobin analysis by capillary zone electrophoresis. J Chromatogr A 709:121–125

    Article  Google Scholar 

  28. Mario N, Baudin B, Bruneel A, Janssens J, Vaubourdolle M (1999) Capillary zone electrophoresis for the diagnosis of congenital hemoglobinopathies. Clin Chem 45:285–288

    Article  CAS  Google Scholar 

  29. Greene DN, Vaughn CP, Crews BO, Agarwal AM (2015) Advances in detection of hemoglobinopathies. Clin Chim Acta 439:50–57

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Nebraska, Lincoln, NE, USA

    David S. Hage

Authors
  1. David S. Hage
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to David S. Hage .

Editor information

Editors and Affiliations

  1. Department of Pharmaceutics, School of Pharmacy, Virginia Commonwealth University, Richmond, VA, USA

    Terry M. Phillips

Rights and permissions

Reprints and permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Hage, D.S. (2019). An Overview of CE in Clinical Analysis. In: Phillips, T.M. (eds) Clinical Applications of Capillary Electrophoresis. Methods in Molecular Biology, vol 1972. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9213-3_1

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-9213-3_1

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9212-6

  • Online ISBN: 978-1-4939-9213-3

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation