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High-Content Imaging of Immunofluorescently Labeled TRPV1-Positive Sensory Neurons

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TRP Channels

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1987))

Abstract

Studying TRP channel expressing nociceptors requires the identification of the respective subpopulations as well as the quantification of dynamic cellular events. However, the heterogeneity of sensory neurons and associated nonneuronal cells demands the analysis of large numbers of cells to reflect the distribution of entire populations. Here we report a detailed workflow how to apply high-content screening (HCS) microscopy to signaling events in TRPV1-positive neurons as well as an approach to use the selective elimination of TRPV1 positive cells from dissociated rat sensory ganglia as base for transcriptomic analysis of TRPV1-positive cells and/or as control for TRPV1 antibody specificity.

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Author information

Authors and Affiliations

  1. Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany

    Jörg Isensee & Tim Hucho

Authors
  1. Jörg Isensee
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  2. Tim Hucho
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Corresponding author

Correspondence to Tim Hucho .

Editor information

Editors and Affiliations

  1. Instituto de Biología, Molecular y Celular, Universidad Miguel Hernández, Elche, Alicante, Spain

    Antonio Ferrer-Montiel

  2. Experimental Anesthesiology and Pain Research, Department of Anesthesiology and Intensive Care Medicine, University Hospital Cologne, University of Cologne, Cologne, Germany

    Tim Hucho

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Isensee, J., Hucho, T. (2019). High-Content Imaging of Immunofluorescently Labeled TRPV1-Positive Sensory Neurons. In: Ferrer-Montiel, A., Hucho, T. (eds) TRP Channels. Methods in Molecular Biology, vol 1987. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9446-5_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9446-5_8

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9445-8

  • Online ISBN: 978-1-4939-9446-5

  • eBook Packages: Springer Protocols

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