Abstract
Studies over the last decade on characterization of the major histocompatibility complex (MHC) class I antigen presentation pathway have highlighted the importance of antigen processing, peptide transport, peptide trimming, and peptide selection as key stages for the development of optimal peptide repertoires that are presented by MHC class I molecules to cytotoxic T lymphocytes (CTLs). The study of these stages and how they are regulated, is fundamental for progress in understanding the adaptive immune system. Here we describe an in vitro assay monitoring peptide trimming by the human endoplasmic reticulum amino peptidases 1 (ERAP1) and ERAP2 (ERAPs) as a tool to characterize trimming events and gain a better understanding of the role and function of ERAPs in peptide repertoire development. Specifically, our assay allows for monitoring trimming of free but also of MHC I-bound peptides which may reflect the physiological situation best.
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Acknowledgments
This work was supported by the Boehringer Ingelheim Foundation (to M.W.), the Fritz-Thyssen-Foundation (to PvE), and the National Institutes of Allergy and Infectious Diseases Grants AI108546 and AI114467 (to M.B.).
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Weimershaus, M., Evnouchidou, I., Li, L., van Endert, P., Bouvier, M. (2019). Trimming of MHC Class I Ligands by ERAP Aminopeptidases. In: van Endert, P. (eds) Antigen Processing. Methods in Molecular Biology, vol 1988. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9450-2_3
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DOI: https://doi.org/10.1007/978-1-4939-9450-2_3
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Publisher Name: Humana, New York, NY
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Online ISBN: 978-1-4939-9450-2
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