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Purification of Native Flagellin

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Proteus mirabilis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2021))

Abstract

Flagella are effective organelles of locomotion and one of several virulence factors in Proteus mirabilis. To study their properties and role in virulence, we describe a protocol to extract and purify the native flagellin of P. mirabilis. Purified flagellin can be visualized by SDS-PAGE or immunoblot and is suitable for downstream applications such as immunization.

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Author information

Authors and Affiliations

  1. Departamento de Microbiología, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay

    María José González, Victoria Iribarnegaray, Pablo Zunino & Paola Scavone

Authors
  1. María José González
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  2. Victoria Iribarnegaray
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  3. Pablo Zunino
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  4. Paola Scavone
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Editor information

Editors and Affiliations

  1. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA

    Melanie M. Pearson

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González, M.J., Iribarnegaray, V., Zunino, P., Scavone, P. (2019). Purification of Native Flagellin. In: Pearson, M. (eds) Proteus mirabilis. Methods in Molecular Biology, vol 2021. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9601-8_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9601-8_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9600-1

  • Online ISBN: 978-1-4939-9601-8

  • eBook Packages: Springer Protocols

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