Abstract
Identification of protein ubiquitination sites is very important for the functional analysis of a targeted protein. Sample preparation before LC-MS/MS assay is essential for this experimental process. Here, we describe two efficient methods for preparing samples for identifying ubiquitination sites in plant proteins by LC-MS/MS.
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Acknowledgments
This work was supported by grant 2016YFA0500500 from the National Key R&D Program of China.
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Yu, F., Xie, Q. (2019). Approaches to Identify Protein Ubiquitination Sites in Plants. In: Hiltbrunner, A. (eds) Phytochromes. Methods in Molecular Biology, vol 2026. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9612-4_6
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DOI: https://doi.org/10.1007/978-1-4939-9612-4_6
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