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Fast NMR Methods for Identification of Resonances and Metabolic Pathways

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NMR-Based Metabolomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2037))

Abstract

High-throughput analysis of NMR data in metabolomics involves both rapid data acquisition and analysis. We describe here a data collection and analysis protocol, which enables fast multidimensional NMR data acquisition and automated analysis of NMR spectra to rapidly identify the metabolites and assign them to active metabolic pathways in the system.

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Acknowledgments

The high-field NMR facility at NMR Research Centre, Indian Institute of Science, supported by the Department of Science and Technology (India) is gratefully acknowledged. HSA acknowledges research grant from SERB (EMR/2016/003181).

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Author notes

  1. Abhinav Dubey

    Present address: Department of Biochemistry and Molecular Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA

  2. Shivanand M. Pudakalakatti

    Present address: Department of Cancer Systems Imaging, University of Texas MD Anderson Cancer Center, Houston, TX, USA

Authors and Affiliations

  1. NMR Research Centre, Indian Institute of Science, Bangalore, India

    Abhinav Dubey, Shivanand M. Pudakalakatti & Hanudatta S. Atreya

Authors
  1. Abhinav Dubey
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  2. Shivanand M. Pudakalakatti
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  3. Hanudatta S. Atreya
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Corresponding author

Correspondence to Hanudatta S. Atreya .

Editor information

Editors and Affiliations

  1. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, WA, USA

    G. A. Nagana Gowda

  2. Northwest Metabolomics Research Center, Mitochondria and Metabolism Center, Department of Anesthesiology and Pain Medicine, University of Washington, Fred Hutch Cancer Research Center, Seattle, WA, USA

    Daniel Raftery

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Dubey, A., Pudakalakatti, S.M., Atreya, H.S. (2019). Fast NMR Methods for Identification of Resonances and Metabolic Pathways. In: Gowda, G., Raftery, D. (eds) NMR-Based Metabolomics. Methods in Molecular Biology, vol 2037. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9690-2_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9690-2_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9689-6

  • Online ISBN: 978-1-4939-9690-2

  • eBook Packages: Springer Protocols

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