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The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology

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Theranostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2054))

Abstract

Metastasis is responsible for 90% of cancer-related deaths. The study of circulating tumor cells (CTCs) enables the study of the units of disease responsible for the process of metastasis. While the biology of the primary tissue is relatively known, little is understood about the cells en route to distant sites. Here we describe the isolation of CTCs using the spiral microfluidic technology for the efficient sorting of CTCs from head and neck cancer (HNC) patient blood samples. Furthermore, the molecular characterization of CTCs can aid in stratifying patients for targeted therapy such as immunotherapy, which is having a profound impact in the treatment of metastatic HNC.

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Acknowledgments

This chapter was supported by the Translational Research Institute (TRI) Spore Grant.

Author information

Authors and Affiliations

  1. The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Arutha Kulasinghe

  2. The School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW, Australia

    Arutha Kulasinghe & Majid Ebrahimi Warkiani

  3. The School of Biomedical Sciences, Institute of Health and Biomedical Innovation, Queensland University of Technology (QUT), Kelvin Grove, QLD, Australia

    Chamindie Punyadeera

  4. Translational Research Institute, Brisbane, QLD, Australia

    Chamindie Punyadeera

Authors
  1. Arutha Kulasinghe
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  2. Majid Ebrahimi Warkiani
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  3. Chamindie Punyadeera
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Corresponding author

Correspondence to Chamindie Punyadeera .

Editor information

Editors and Affiliations

  1. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Jyotsna Batra

  2. School of Biomedical Sciences, Institute of Health and Biomedical Innovation (IHBI), Translational Research Institute, Queensland University of Technology (QUT), Brisbane, QLD, Australia

    Srilakshmi Srinivasan

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Kulasinghe, A., Warkiani, M.E., Punyadeera, C. (2019). The Isolation and Characterization of Circulating Tumor Cells from Head and Neck Cancer Patient Blood Samples Using Spiral Microfluidic Technology. In: Batra, J., Srinivasan, S. (eds) Theranostics. Methods in Molecular Biology, vol 2054. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9769-5_8

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  • DOI: https://doi.org/10.1007/978-1-4939-9769-5_8

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9768-8

  • Online ISBN: 978-1-4939-9769-5

  • eBook Packages: Springer Protocols

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