Abstract
Staphylococcal protein A (SPA) consists of Fc-partial-region, X-region, and C-terminal. Spa typing is a genotyping method based on the presence of gene polymorphism in a variable number of 24 bp repeat sequences in the X-region. Spa typing allows highly discriminatory and rapid characterization and prediction of multilocus sequence typing (MLST), ribotyping, and pulsed-field gel electrophoresis (PFGE). DNA sequence-based approaches are becoming more frequently used because of the ease with which sequence data can be transferred between laboratories via the Internet.
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Acknowledgment
We thank Yingjie Wu and Kai Wang at AuGCT DNA-SYN Biotechnology Co. Ltd. (Yangling) for data analysis of the cleaned PCR product, sequencing reaction, and DNA sequencing.
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Wang, X. (2020). Spa Typing of Staphylococcus aureus Isolates. In: Ji, Y. (eds) Methicillin-Resistant Staphylococcus Aureus (MRSA) Protocols. Methods in Molecular Biology, vol 2069. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9849-4_6
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DOI: https://doi.org/10.1007/978-1-4939-9849-4_6
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