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Measuring MIF in Biological Fluids

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Macrophage Migration Inhibitory Factor

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2080))

Abstract

MIF is a key regulator of host immune responses and increased levels secreted from cells, or found circulating systemically, have been implicated in the pathogenesis of many inflammatory and autoimmune disorders. Here, we describe methods for detecting and quantifying extracellular concentrations of MIF in both human- and murine-derived biological samples.

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References

  1. Lang T et al (2015) MIF: implications in the pathoetiology of systemic lupus erythematosus. Front Immunol 6:577

    Article  Google Scholar 

  2. Bloom BR, Bennett B (1966) Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science 153(3731):80–82

    Article  CAS  Google Scholar 

  3. David JR (1966) Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A 56(1):72–77

    Article  CAS  Google Scholar 

  4. Baugh JA, Bucala R (2002) Macrophage migration inhibitory factor. Crit Care Med 30(1 Supp):S27–S35

    Article  CAS  Google Scholar 

  5. Lan HY (2008) Role of macrophage migration inhibition factor in kidney disease. Nephron Exp Nephrol 109(3):e79–e83

    Article  CAS  Google Scholar 

  6. Calandra T, Roger T (2003) Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol 3(10):791–800

    Article  CAS  Google Scholar 

  7. Vincent FB et al (2019) Serum and urinary macrophage migration inhibitory factor (MIF) in primary Sjogren’s syndrome. Joint Bone Spine 86(3):393–395

    Article  Google Scholar 

  8. Vincent FB et al (2018) Analysis of serum macrophage migration inhibitory factor and D-dopachrome tautomerase in systemic sclerosis. Clin Transl Immunol 7(12):e1042

    Article  Google Scholar 

  9. Vincent FB et al (2018) Analysis of urinary macrophage migration inhibitory factor in systemic lupus erythematosus. Lupus Sci Med 5(1):e000277

    Article  Google Scholar 

  10. Vincent FB et al (2019) Effect of storage duration on cytokine stability in human serum and plasma. Cytokine 113:453–457

    Article  CAS  Google Scholar 

  11. Tuck MK et al (2009) Standard operating procedures for serum and plasma collection: early detection research network consensus statement standard operating procedure integration working group. J Proteome Res 8(1):113–117

    Article  CAS  Google Scholar 

  12. Keustermans GC et al (2013) Cytokine assays: an assessment of the preparation and treatment of blood and tissue samples. Methods 61(1):10–17

    Article  CAS  Google Scholar 

  13. Thomas CE et al (2010) Urine collection and processing for protein biomarker discovery and quantification. Cancer Epidemiol Biomark Prev 19(4):953–959

    Article  CAS  Google Scholar 

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Correspondence to Tali Lang .

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Vincent, F.B., Lang, T. (2020). Measuring MIF in Biological Fluids. In: Harris, J., Morand, E. (eds) Macrophage Migration Inhibitory Factor. Methods in Molecular Biology, vol 2080. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9936-1_5

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  • DOI: https://doi.org/10.1007/978-1-4939-9936-1_5

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9935-4

  • Online ISBN: 978-1-4939-9936-1

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