Abstract
In Northern analysis the presence of specific RNA transcripts is detected and their quantity can be estimated. RNA is separated using denaturing agarose gel electrophoresis and is subsequently transferred and fixed to a solid support, such as a nitrocellulose filter. When labeled probes are hybridized to these immobilized RNA molecules, their presence can be visualized by autoradiography. Here we describe Northern hybridization using radioactively labeled riboprobes to show circadian expression of endogenous sense and antisense frequency RNA in the filamentous fungus Neurospora crassa.
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Kramer, C., Crosthwaite, S.K. (2007). Northern Analysis of Sense and Antisense frequency RNA in Neurospora crassa . In: Rosato, E. (eds) Circadian Rhythms. Methods in Molecular Biology™, vol 362. Humana Press. https://doi.org/10.1007/978-1-59745-257-1_23
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DOI: https://doi.org/10.1007/978-1-59745-257-1_23
Publisher Name: Humana Press
Print ISBN: 978-1-58829-417-3
Online ISBN: 978-1-59745-257-1
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