Abstract
Post-transcriptional control makes an important contribution to shaping transcript profiles of circadi- anly regulated genes. In Ambidopsis thaliana, the clock-regulated glycine-rich RNA-binding protein ATGKP7 oscillates with a 24-h rhythm and transmits the rhythmicity generated by the central oscillator within the cell. ATGRP7 negatively auto-regulates its own expression at the post-transcriptional level. In response to an elevated protein level, a shift to a cryptic 5' splice site within the intron occurs, leading to an unproductively spliced transcript that rapidly vanishes due to its short half-life. This feedback regulation relies on direct binding of the RNA-binding protein to its own RNA. Here we describe the analysis of RNA-protein interaction in vitro employing recombinant RNA-binding protein and 32P-labelled in vitro transcripts or synthetic RNA oligoribonucleotides comprising the binding site under study.
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Schöning, J., Staiger, D. (2009). RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System. In: Pfannschmidt, T. (eds) Plant Signal Transduction. Methods in Molecular Biology, vol 479. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-289-2_21
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DOI: https://doi.org/10.1007/978-1-59745-289-2_21
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