Abstract
The study of protein-protein and protein-DNA interactions is critical to understand biological processes. This article presents the methodology to create peptide microarrays in situ for the high-throughput screening of complex biomolecules. The in situ ink-jet peptide synthesis results in a conservation of costly reagent and amino acids, whereas providing a means to produce denser peptide arrays. A smaller amount of test sample is required to observe interaction when using these high-density peptide arrays.
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References
Min, D. H. and Mrksich, M. (2004) Peptide arrays: towards routine implementation. Curr. Opin. Chem. Biol. 8, 554–558.
Panicker, R. C., Huang, X., and Shao, Q. Y. (2004) Recent advances in peptide-based microarray technologies. Comb. Chem. High Throughput Screen. 7, 547–556.
Fujii, K., Zhu, G., Liu, Y., et al. (2005) Kinase peptide specificity: improved determination and relevance to protein phosphorylation. Proc. Natl. Acad. Sci. USA 101, 13,744–13,749.
Sun, H., Low, K. E., Woo, S., et al. (2005) Real-time protein kinase assay. Anal. Chem. 77, 2043–2049.
Falsey, J. R., Renil, M., Park, S., Li, S., and Lam, K. S. (2001) Peptide and small molecule microarray for high throughput cell adhesion and functional assays. Bioconjug. Chem. 12, 346–353.
Thorpe, D. S., Yeoman, H., Chan, A. W., Krchnak, V., Lebl, M., and Felder, S. (1999) Combinatorial chemistry reveals a new motif that binds the platelet fibrinogen receptor, gpIIbIIIa. Biochem. Biophys. Res. Commun. 256, 537–541.
Bowditch, R. D., Tani, P., Foong, K. C., and McMilan, R. (1996) Characterization of autoantigenic epitopes on platelet glycoprotein Iib/IIIa using random peptide libraries, Blood 15, 4579–4584.
Swan, E. E., Popat, K. C., and Desai, T. A. (2005) Peptide-immobilized nanoporous alumina membranes for enhanced osteoblast adhesion. Biomaterials 26, 1969–1976.
Spear, M. A., Breakefield, X. O., Beltzer, J., et al. (2001) Isolation, characterization, and recovery of small peptide phage display epitopes selected against viable malignant glioma cells. Cancer Gene Ther. 8, 506–511.
Houghten, R. A. (1985) General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids, Proc. Natl. Acad. Sci. USA 82, 5131–5135.
Reid, J., Betney, R., Watt, K., and McEwan, I. J. (2003) The androgen receptor transactivation domain: the interplay between protein conformation and protein-protein interaction. Biochem. Soc. 31, 1042–1046.
Huang, X., Pieczko, M. E., and Long, E. C. (1999) Combinatorial optimization of the DNA cleaving Ni(II) x Xaa-Xaa-His metallotripeptide domain. Biochemistry 38, 2160–2166.
Pennington, M., Lam, K., and Cress, A. E. (1996) The use of combinatorial library method to isolate human tumor cell adhesion peptides. Mol. Diversity 2, 19–28.
Frank, R. (1992) Spot-Synthesis: an easy technique for the positionally addressable, parallel chemical synthesis on a membrane support. Tetrahedron 48, 9217–9232.
(1999) Sigma-Genosys custom SPOT’s technical manual, ver 3, Sigma-Genosys, The Woodlands, TX, p. 51.
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© 2007 Humana Press Inc., Totowa, NJ
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Antohe, B.V., Cooley, P.W. (2007). In Situ Synthesis of Peptide Microarrays Using Ink-Jet Microdispensing. In: Rampal, J.B. (eds) Microarrays. Methods in Molecular Biology™, vol 381. Humana Press. https://doi.org/10.1007/978-1-59745-303-5_15
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DOI: https://doi.org/10.1007/978-1-59745-303-5_15
Publisher Name: Humana Press
Print ISBN: 978-1-58829-589-7
Online ISBN: 978-1-59745-303-5
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