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Detection of the MDR1 P-Glycoprotein Expression and Function

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Monoclonal Antibodies

Part of the book series: Methods in Molecular Biology ((MIMB,volume 378))

Abstract

Acquired and intrinsic multidrug resistance is the major reason for the failure of anticancer chemotherapy. The most important component of clinical multidrug resistance is mediated by P-glycoprotein (Pgp), an ABC transporter encoded by the MDR1 gene and expressed on the membrane of tumor and normal cells. Sensitive and reproducible detection of Pgp expression and function are critical for the development of new MDR1 drugs and clinical protocols aimed at modulating Pgp-mediated multidrug resistance. The most commonly used methods for detecting Pgp distribution and functional activity have major flaws when used in routine clinical diagnostics. In this chapter, we describe and compare these techniques and introduce a new method for simultaneous detection of Pgp expression and function—the UIC2 Shift assay.

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Author information

Authors and Affiliations

  1. Department of Research and Development, Oncotech Inc., Tustin, CA

    Eugene Mechetner

  2. Department of Radiological Sciences, College of Medicine, University of California-Irvine, Irvine, CA

    Eugene Mechetner

Authors
  1. Eugene Mechetner
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Editor information

Editors and Affiliations

  1. Department of Hematopathology, Quest Diagnostics Nichols Institute, San Juan Capistrano, CA

    Maher Albitar

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© 2007 Humana Press Inc., Totowa, NJ

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Mechetner, E. (2007). Detection of the MDR1 P-Glycoprotein Expression and Function. In: Albitar, M. (eds) Monoclonal Antibodies. Methods in Molecular Biology, vol 378. Humana Press. https://doi.org/10.1007/978-1-59745-323-3_13

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  • DOI: https://doi.org/10.1007/978-1-59745-323-3_13

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-567-5

  • Online ISBN: 978-1-59745-323-3

  • eBook Packages: Springer Protocols

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