Abstract
The diet known as caloric restriction (CR) has been known for 70 yr to extend the life span of rodents (1). CR can also extend life span in a broad range of other species as well, from unicellular organisms (2,3), to invertebrates (4) and most likely primates, as well (5). The budding yeast Saccharomyces cerevisiae is a useful model for the study of pathways that determine life span in response to dietary intake. Here, we describe how to calorically restrict yeast, the methods used to determine the replicative life span of single yeast “mother” cells and measure recombination frequency at the rDNA locus, and how to isolate and analyze the circular forms of DNA known as extrachromosomal rDNA circles (ERCs), which are a major cause of aging in S. cerevisiae (6–8).
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
McCay, C. M., Maynard, L. A., Sperling, G., and Barnes, L. L. (1975) Retarded growth, life span, ultimate body size and age changes in the albino rat after feeding diets restricted in calories. Nutr. Rev. 33, 241–243.
Jiang, J. C., Jaruga, E., Repnevskaya, M. V., and Jazwinski, S. M. (2000) An intervention resembling caloric restriction prolongs life span and retards aging in yeast. FASEB J. 14, 2135–2137.
Lin, S. J., Defossez, P. A., and Guarente, L. (2000) Requirement of NAD and SIR2 for life-span extension by calorie restriction in Saccharomyces cerevisiae. Science 289, 2126–2128.
Rogina, B., and Helfand, S. L. (2004) Sir2 mediates longevity in the fly through a pathway related to calorie restriction. Proc. Natl. Acad. Set USA 101, 15,998–16,003.
Lane, M. A., Mattison, J., Ingram, D. K., and Roth, G. S. (2002) Caloric restriction and aging in primates: relevance to humans and possible CR mimetics. Micros. Res. Tech. 59, 335–338.
Petes, T. D. (1980) Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell 19, 765–774.
Sinclair, D. A. and Guarente, L. (1997) Extrachromosomal rDNA circles—a cause of aging in yeast. Cell 91, 1033–1042.
Defossez, P. A., Prusty, R., Kaeberlein, M., et al. (1999) Elimination of replication block protein Fob1 extends the life span of yeast mother cells. Mol. Cell 3, 447–455.
Bitterman, K. J., Medvedik, O., and Sinclair, D. A. (2003) Longevity regulation in Saccharomyces cerevisiae: linking metabolism, genome stability, and hetero-chromatin. Micro. Mol. Biol. Rev. 67, 376–399.
Fabrizio, P. and Longo, V. D. (2003) The chronological life span of Saccharomyces cerevisiae. Aging Cell 2, 73–81.
Barton, A. A. (1950) Some aspects of cell division in saccharomyces cerevisiae. J. Gen. Microbiol. 4, 84–86.
Sinclair, D. A. and Guarente, L. (1997) Extrachromosomal rDNA circles—a cause of aging in yeast. Cell 91(7), 1033–1042.
Petes, T. D. (1980) Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes. Cell 19, 765–774.
Falcon, A. A. and Aris, J. P. (2003) Plasmid accumulation reduces life span in Saccharomyces cerevisiae. J. Biol. Chem. 278(43), 41,607–41,617.
Lamming, D. W., Latorre-Esteves, M., Medvedik, O., et al. (2005) HST2 mediates SIR2-independent life-span extension by calorie restriction. Science 309, 1861–1864.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2007 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Medvedik, O., Sinclair, D.A. (2007). Caloric Restriction and Life Span Determination of Yeast Cells. In: Tollefsbol, T.O. (eds) Biological Aging. Methods in Molecular Biology™, vol 371. Humana Press. https://doi.org/10.1007/978-1-59745-361-5_9
Download citation
DOI: https://doi.org/10.1007/978-1-59745-361-5_9
Publisher Name: Humana Press
Print ISBN: 978-1-58829-658-0
Online ISBN: 978-1-59745-361-5
eBook Packages: Springer Protocols