Abstract
Gas chromatography–mass spectrometry (GC-MS)-based analysis is an accurate and sensitive method to quantify plant hormones. This method is commonly used for analysis of low-molecular-weight compounds, such as abscisic acid (ABA), gibberellins (GAs), auxins, and brassinosteroids. Procedures are composed of four major steps: extraction, fractionation, derivatization, and detection. Here, we describe a protocol for quantification of ABA and GAs.
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References
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© 2009 Humana Press, a part of Springer Science+Business Media, LLC
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Okamoto, M., Hanada, A., Kamiya, Y., Yamaguchi, S., Nambara, E. (2009). Measurement of Abscisic Acid and Gibberellins by Gas Chromatography/Mass Spectrometry. In: Cutler, S., Bonetta, D. (eds) Plant Hormones. Methods in Molecular Biology, vol 495. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-477-3_5
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DOI: https://doi.org/10.1007/978-1-59745-477-3_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-934115-32-9
Online ISBN: 978-1-59745-477-3
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