Abstract
The authors developed a technique to apply high hydrostatic pressure to giant unilamellar vesicles and to directly observe the consequent structural changes with two-photon fluorescence microscopy imaging using high numerical aperture oil-immersion objectives. The data demonstrate that high pressure has a dramatic effect on the shape of the vesicles, and both fluidity and homogeneity of the membrane.
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Celli, A., Lee, C.Y.C., Gratton, E. (2007). Fluorescence Microscopy to Study Pressure Between Lipids in Giant Unilamellar Vesicles. In: Dopico, A.M. (eds) Methods in Membrane Lipids. Methods in Molecular Biology™, vol 400. Humana Press. https://doi.org/10.1007/978-1-59745-519-0_22
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DOI: https://doi.org/10.1007/978-1-59745-519-0_22
Publisher Name: Humana Press
Print ISBN: 978-1-58829-662-7
Online ISBN: 978-1-59745-519-0
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