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Slice Blotting

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Protein Blotting and Detection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 536))

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Summary

Slice blotting is a technique for recording the spatial distribution of extracellular signaling molecules released from thin slices of living tissue. Slices are positioned on the surface of a membrane that can trap secreted substances diffusing from the tissue. The pattern of membrane-bound antigens is subsequently visualized by immunoblotting.

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References

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Author information

Authors and Affiliations

  1. Monell Chemical Senses Center, 3500 Market St, Philadelphia, PA, 19104-3308, USA

    Graeme Lowe

Authors
  1. Graeme Lowe
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Corresponding author

Correspondence to Graeme Lowe .

Editor information

Editors and Affiliations

  1. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    Biji T. Kurien

  2. Oklahoma Medical Research Foundation, NE. 13th Street 825, Oklahoma City, 73104, U.S.A.

    R. Hal Scofield

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Lowe, G. (2009). Slice Blotting. In: Kurien, B., Scofield, R. (eds) Protein Blotting and Detection. Methods in Molecular Biology, vol 536. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-59745-542-8_9

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  • DOI: https://doi.org/10.1007/978-1-59745-542-8_9

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-934115-73-2

  • Online ISBN: 978-1-59745-542-8

  • eBook Packages: Springer Protocols

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