Summary
Membrane proteins are rarely identified in two-dimensional electrophoretic (2-DE) proteomics maps. This is because of low abundance, poor solubility, and inherent hydrophobicity. In this study, membrane preparations from the Gram-positive bacterium Streptococcus mutans were isolated from protoplasts and by mechanical grinding. Membrane proteins were extracted using a mixture of trifluroethanol and chloroform, solubilized using highly chaotropic buffer containing ASB-14 and Triton X-100 and subjected to two-dimensional gel electrophoresis.
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© 2008 Humana Press, a part of Springer Science+Business Media, LLC
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Zuobi-Hasona, K., Brady, L.J. (2008). Isolation and Solubilization of Cellular Membrane Proteins from Bacteria. In: Posch, A. (eds) 2D PAGE: Sample Preparation and Fractionation. Methods in Molecular Biology™, vol 425. Humana Press. https://doi.org/10.1007/978-1-60327-210-0_23
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DOI: https://doi.org/10.1007/978-1-60327-210-0_23
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Print ISBN: 978-1-60327-209-4
Online ISBN: 978-1-60327-210-0
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