Abstract
m-Maleimidobenzoyl-N-hydroxysuccinimide ester (MBS) is a heterobifunctional agent that links a thiol group to an amino group at neutral pH. For peptide conjugation, the peptide usually provides the thiol group in the form of a cysteine residue, whereas the carrier provides amino groups in the form of lysine residues. The reaction proceeds in two steps. First the carrier is activated by reaction of its amino group with the succinimide moiety. Then the thiol group on the peptide reacts with the maleimide moiety of the activated carrier.
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References
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© 1996 Humana Press Inc., Totowa, NJ
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Carter, J.M. (1996). Conjugation of Peptide to Carrier Proteins via m-Maleimidobenzoyl-N-Hydroxysuccinimide Ester (MBS). In: Walker, J.M. (eds) The Protein Protocols Handbook. Springer Protocols Handbooks. Humana Press. https://doi.org/10.1007/978-1-60327-259-9_118
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DOI: https://doi.org/10.1007/978-1-60327-259-9_118
Publisher Name: Humana Press
Print ISBN: 978-0-89603-338-2
Online ISBN: 978-1-60327-259-9
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