Abstract
The reader may be surprised to find a chapter about a Western Blotting technique in a book dealing with affinity chromatography. However, the method described here combines two steps that are characteristic of immuno affinity chromatography. One is the preparation of monospecific antibodies against a particular antigen and the second is the testing of their reactivity with the same or different antigens from any source of antigenic material.
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References
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Hammerl P., et al. (1992) A method for the detection of serologically Crossreacting antigens both within and between protein mixtures: the Western Cross blot. J. Immunol. Methods 151, 299.
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© 2000 Humana Press Inc.
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Hammerl, P., Hartl, A., Freund, J., Thalhamer, J. (2000). Western Cross Blot. In: Bailon, P., Ehrlich, G.K., Fung, WJ., Berthold, W. (eds) Affinity Chromatography. Methods in Molecular Biology, vol 147. Humana Press. https://doi.org/10.1007/978-1-60327-261-2_16
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DOI: https://doi.org/10.1007/978-1-60327-261-2_16
Publisher Name: Humana Press
Print ISBN: 978-0-89603-694-9
Online ISBN: 978-1-60327-261-2
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