Abstract
Many organisms, including humans, have many more proteins than are actually coded for by their genes. This discrepancy is partially explained by the existence of alternative transcripts produced by the same gene. Multiple isoforms of the same gene sometimes perform completely different functions, and as such, knowing the sequence of one of the transcripts is not enough. Rapid Amplification of cDNA Ends (RACE) provides an inexpensive and powerful tool to quickly identify alternative transcripts of a gene when the partial or complete sequence of only one transcript is known. In the following sections, we outline details for rapid amplification of 5’ and 3’ cDNA ends using the “New Race” technique.
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Acknowledgments
This work was supported by NIH awards GM071520 and DK64166 to MAF and an NIH T32 Medical Scientist Training Grant and NIH F31-DK082280 awards to OY.
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Yeku, O., Scotto-Lavino, E., Frohman, M.A. (2009). Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE). In: Park-Sarge, OK., Curry, T. (eds) Molecular Endocrinology. Methods in Molecular Biology, vol 590. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60327-378-7_18
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DOI: https://doi.org/10.1007/978-1-60327-378-7_18
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