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Preparation of Tissue and Sections

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Enzymatic Analysis

Part of the book series: Biological Methods ((BM))

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Abstract

The following portion of this book represents a coherent system of quantitative analysis for histochemical purposes. It derives from the original system of Linderstrom-Lang and Holter (1), but differs in several significant details. In the original methodology of the Carlsberg Laboratory, serial frozen sections were cut from a uniform tissue block. Alternate sections were either analyzed, or fixed and stained for histological control. This procedure has been further developed and exploited with great success by David Glick, and is fully described in book form (2).

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References

  1. Linderstrom-Lang, K., Holter, H., and Ohlsen, A. S. (1935) Studies on enzymatic histochemistry XIII. The distribution of enzymes in the stomach of pigs. Compt.-Rend. Lab. Carlsberg, Serie Chim. 20, 66–125.

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  2. Glick, D. (1961,1963) Quantitive Chemical Techniques of Histo-and Cytochemistry, vols. 1 and 2 (Wiley [Interscience]), New York.

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  3. Cole, B. R., Boylan, J. G., Bross, T. E., Burch, H. B., and Lowry, O. H. (1988) Progressive enzyme changes within anatomically defined segments of rat nephron: Demonstration of anew technique. J. Histochem. Cytochem. 36, 285–289.

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  9. Lowry, C. V., Kimmey, J. S., Felder, S., Chi, M. M.-Y., Kaiser, K. K., Passonneau, P. N., Kirk, K. A., and Lowry, O. H. (1978) Enzyme patterns in single human muscle fibers. J. Biol. Chem. 253, 8269–8277.

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© 1993 The Humana Press Inc.

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Passonneau, J.V., Lowry, O.H. (1993). Preparation of Tissue and Sections. In: Enzymatic Analysis. Biological Methods. Humana Press. https://doi.org/10.1007/978-1-60327-407-4_9

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  • DOI: https://doi.org/10.1007/978-1-60327-407-4_9

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-238-5

  • Online ISBN: 978-1-60327-407-4

  • eBook Packages: Springer Book Archive

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