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Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model

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Heterologous Expression of Membrane Proteins

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 601))

Abstract

In the process of selecting an appropriate host for the heterologous expression of functional eukaryotic membrane proteins, Drosophila S2 cells, although not yet fully explored, appear as a valuable alternative to mammalian cell lines or other virus-infected insect cell systems. This nonlytic, plasmid-based system actually combines several major physiological and bioprocess advantages that make it a highly potential and scalable cellular tool for the production of membrane proteins in a variety of applications, including functional characterization, pharmacological profiling, molecular simulations, structural analyses, or generation of vaccines. We present here a series of protocols and hints that would serve the successful expression of membrane proteins in S2 cells, using an enhanced green fluorescent protein (EGFP)/G protein-coupled receptor (EGFP-GPCR) as a model.

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Authors and Affiliations

  1. Dpt Récepteurs et des Protéines Membranaires, Illkirch, France

    Karl Brillet & Renaud Wagner

  2. Laboratório de Imunologia Viral, Instituto Butantan, São Paulo, Brasil

    Carlos A. Pereira

Authors
  1. Karl Brillet
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  2. Carlos A. Pereira
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  3. Renaud Wagner
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Corresponding author

Correspondence to Renaud Wagner .

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Editors and Affiliations

  1. CNRS - UMR 6543, Université Nice, parc Valrose, Nice CX 02, 06108, France

    Isabelle Mus-Veteau

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Brillet, K., Pereira, C.A., Wagner, R. (2010). Expression of Membrane Proteins in Drosophila Melanogaster S2 Cells: Production and Analysis of a EGFP-Fused G Protein-Coupled Receptor as a Model. In: Mus-Veteau, I. (eds) Heterologous Expression of Membrane Proteins. Methods in Molecular Biology™, vol 601. Humana Press. https://doi.org/10.1007/978-1-60761-344-2_8

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  • DOI: https://doi.org/10.1007/978-1-60761-344-2_8

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-343-5

  • Online ISBN: 978-1-60761-344-2

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