Abstract
Photo-activatable (caged) probes are powerful research tools for biological investigation. The superb maneuverability of a light beam allows researchers to activate caged probes with pinpoint accuracy. Recent developments in caging chemistry and two-photon excitation technique further enhance our capability to perform photo-uncaging with even higher spatial and temporal resolution, offering new photonic approaches to study cell signaling dynamics in greater detail. Here we present a sample method that combines the techniques of photo-activation and digital fluorescence microscopy to assay an important class of intracellular receptors for the second messenger D-myo-inositol 1,4,5-trisphosphate (Ins(1,4,5)P3, or IP3). The imaging assay is performed in fully intact living cells using a caged and cell membrane permeable ester derivative of IP3, cm-IP3/PM.
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Li, WH. (2010). Photo-Activatable Probes for the Analysis of Receptor Function in Living Cells. In: Papkovsky, D. (eds) Live Cell Imaging. Methods in Molecular Biology, vol 591. Humana Press. https://doi.org/10.1007/978-1-60761-404-3_6
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DOI: https://doi.org/10.1007/978-1-60761-404-3_6
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