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Preventing Immune Rejection Through Gene Silencing

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RNA Interference

Part of the book series: Methods in Molecular Biology ((MIMB,volume 623))

Abstract

Dendritic cells (DCs) comprise a family of professional antigen-presenting cells responsible for the induction of primary immune responses. DCs are also important for the induction of immunological tolerance. Recent research has revealed that DC maturation is associated with activation of the NF-κB pathway. RelB, one of the five families of Rel proteins involved in the NF-κB pathway, plays a critical role in coordinating the terminal stages of DC maturation and has the ability to induce optimal Th1 T cell responses. DCs generated from mouse bone marrow can be silenced using siRNA specific for the target gene. Silencing RelB in DCs will result in the generation of immunoregulatory dendritic cells that inhibit allogenic T cell responses. The KLH-specific T cell response should also be inhibited after the RelB siRNA treatment. Furthermore, silencing the RelB gene in DCs can generate regulatory T cells. Administering donor-derived RelB-silencing DCs can prevent allograft rejection in murine heart transplantation.

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Author information

Authors and Affiliations

  1. Departments of Surgery and Pathology, University of Western Ontario, London, ON, Canada

    Xusheng Zhang

  2. Applied Biosystems/Ambion, Austin, TX, USA

    Mu Li

  3. Departments of Surgery, Microbiology and Immunology, and Pathology, University of Western Ontario, London, ON, Canada

    Wei-Ping Min

  4. The Multi-Organ Transplant Program, London Health Sciences Centre, London, ON, Canada

    Wei-Ping Min

  5. Transplantation, Immunity and Regenerative Medicine, Lawson Health Research Institute, London, ON, Canada

    Wei-Ping Min

Authors
  1. Xusheng Zhang
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  2. Mu Li
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  3. Wei-Ping Min
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Corresponding author

Correspondence to Xusheng Zhang .

Editor information

Editors and Affiliations

  1. Dept. Surgery, Pathology & Oncology, University of Western Ontario, Windermere Road 339, London, N6A 5A5, Canada

    Wei-Ping Min

  2. MediStem Laboratories, Inc., E. Cedar Street 2027, Tempe, 85281, USA

    Thomas Ichim

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Zhang, X., Li, M., Min, WP. (2010). Preventing Immune Rejection Through Gene Silencing. In: Min, WP., Ichim, T. (eds) RNA Interference. Methods in Molecular Biology, vol 623. Humana Press. https://doi.org/10.1007/978-1-60761-588-0_23

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  • DOI: https://doi.org/10.1007/978-1-60761-588-0_23

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  • Publisher Name: Humana Press

  • Print ISBN: 978-1-60761-587-3

  • Online ISBN: 978-1-60761-588-0

  • eBook Packages: Springer Protocols

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