Abstract
Tissue profiling and imaging by MALDI mass spectrometry has allowed the direct analysis and localization of biomolecules in tissue. However, due to the in situ nature of this technique, the complexity of tissue, and the need for a chemical matrix in MALDI, the signal recorded can be extremely complex and difficult to assign. Combining ion mobility with matrix-assisted laser desorption/ionization is a very powerful technique for fast separation and analysis of biomolecules in complex mixtures (such as tissue and cells), as isobaric lipid, peptide, and oligonucleotide molecular ions are pre-separated in the mobility cell before mass analysis. Differences in drift time of as much as 30% are obtained in a timescale of hundreds of microseconds. Molecular ions of the same biochemical family fall along trend lines when plotted in 2D plots of mobility drift time as a function of m/z. In this chapter ion mobility MALDI-MS ability to analyze various biomolecules in tissue, that is, lipids and proteins, as well as its ability to separate species from all of the major phospholipid classes from tissue and extracts, the effects that radyl chain length, degree of unsaturation, head group composition have upon their ion’s cross section in the gas phase, and how it can be used not only to distinguish them from other biochemical groups in a mixture but also to differentiate them from other lipid species will be illustrated.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Holthuis, J. C. M., Levine, T. P. (2005) Lipid traffic: floppy drives and a superhighway. Nat Rev Mol Cell Biol, 6, 209–220.
Waston, A. D. (2006) Lipidomics: a global approach to lipid analysis in biological systems. J Lipid Res, 47, 2101–2111.
van Meer, G. (2005) Cellular lipidomics. EMBO J, 24, 3159–3165.
Wenk, M. R. (2005) The emerging field of lipidomics. Nat Rev Drug Discov, 7, 594–610.
Piomelli, D., Astarita, G., Rapaka, R. (2007) A neuroscientist’s guide to lipidomics. Nat Rev Neurosci, 8, 743–753.
Han, X. (2007) Neurolipidomics: challenges and developments. Front Biosci, 12, 2601–2615.
Gillig, K. J., Ruotolo, B., Stone, E. G., Russell, D. H., Fuhrer, K., Gonin, M., Schultz, J. A. (2000) Coupling high-pressure MALDI with ion mobility/orthogonal time-of-flight mass spectrometry. Anal Chem, 72, 3965–3971.
von Helden, G., Wyttenbach T., Bowers, M. T. (1995) Conformation of macromolecules in the gas phase: use of matrix-assisted laser desorption methods in ion chromatography. Science, 267, 1483–1485.
Baumbach, J. I., Eiceman, G. A. (1999) Ion mobility spectrometry: arriving on site and moving beyond a low profile. Appl Spectrosc, 53, 338A–355A.
McDaniel, E. W., Mason, E. A. The Mobility and Diffusion of Ions in Gases. Wiley, New York, NY, 68–72, 1973.
Collins, D. C., Lee, M. L. (2002) Developments in ion mobility spectrometry-mass spectrometry. Anal Bioanal Chem, 372, 66–73.
Woods, A. S., Ugarov, M., Egan, T., Koomen, J. Gillig, K. J., Fuhrer, K., Gonin, M., Schultz, J. A. (2004) Lipid/peptide/nucleotide separation with MALDI-ion mobility-TOF MS. Anal Chem, 76, 2187–2195.
Jackson, S. N., Ugarov, M., Egan, T., Post, J. D., Langlais, D., Schultz, J. A., Woods, A. S. (2007) MALDI-ion mobility-TOFMS imaging of lipids in rat brain tissue. J. Mass Spectrom, 42, 1093–1098.
McLean, J. A., Ridenour, W. B., Caprioli, R. M. (2007) Profiling and imaging of tissues by imaging ion mobility-mass spectrometry. J Mass Spectrom, 42, 1099–1105.
Trim, P. J., Henson, C. M., Avery, J. L., McEwen, A., Snel, M. F., Claude, E., Marshall, P. S., West, A., Princivalle, A. P., Clench, M. R. (2008) Matrix-assisted laser desorption/ionization-ion mobility separation-mass spectrometry imaging of vinblastine in whole body tissue sections. Anal Chem, 80, 8628–8634.
Schwartz, S. A., Reyzer, M. L., Caprioli, R. M. (2003) Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation. J Mass Spectrom, 38, 699–708.
Jackson S. N., Wang H-Y. J., Woods, A. S., Ugarov M., Egan T., Schultz J. A. (2005) Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI- ion mobility-TOFMS. J Am Soc Mass Spectrom, 16, 133–138.
Agranoff, B. W., Benjamins, J. A., Hajra, A. K. (1999) Basic Neurochemistry Molecular, Cellular and Medical Aspects. 6th ed. Siegel, G. J. et al., Eds., Lippincott Williams & Wilkins, Philadelphia, PA, 47–67.
Jackson, S. N., Ugarov, M., Post, J. D., Egan, T., Langlais, D., Schultz, J. A., Woods, A. S. (2008) A study of phospholipids by ion mobility TOFMS. J Am Soc Mass Spectrom, 19, 1655–1662.
Novikov, A., Caroff, M., Della-Negra, S., Lebeyec, Y., Pautrat, M., Schultz, J. A., Tempez, A., Wang H-Y. J., Jackson, S. N., Woods, A. S. (2004) Matrix-implanted laser desorption/ionization mass spectrometry. Anal Chem, 76, 7288–7293.
Tempez A., Ugarov, M., Egan, T., Schultz, J. A., Novikov, A., Della-Negra, S., Lebeyec, Y., Pautrat, M., Caroff, M., Smentkowski, V. S., Wang, H-Y. J., Jackson, S. N., Woods, A. S. (2005) Matrix implanted laser desorption ionization (MILDI) combined with ion mobility-mass spectrometry for bio-surface analysis. J Proteome Res, 4, 540–545.
Acknowledgments
This research was supported by the Intramural Research Program of the National Institute on Drug Abuse, NIH. The authors thank the Office of National Drug Control Policy (ONDCP) for instrumentation funding, without which this and other projects could not have been accomplished and Dr. J Albert Schultz and Tom Egan (Ionwerks, Inc.) for assistance with the ion mobility mass spectrometer and the imaging computer software.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2010 Springer Science+Business Media, LLC
About this protocol
Cite this protocol
Woods, A.S., Jackson, S.N. (2010). The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids. In: Rubakhin, S., Sweedler, J. (eds) Mass Spectrometry Imaging. Methods in Molecular Biology, vol 656. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-746-4_5
Download citation
DOI: https://doi.org/10.1007/978-1-60761-746-4_5
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-745-7
Online ISBN: 978-1-60761-746-4
eBook Packages: Springer Protocols