Abstract
Polyclonal antibodies are derived from multiple B-cell clones that have differentiated into antibody-producing plasma cells in response to an immunogen. Polyclonal antibodies raised against a single molecular species of antigen recognize multiple epitopes on a target molecule resulting in signal amplification in indirect immunoassays, including immuno-electron microscopy. In this chapter, we present a basic procedure to generate polyclonal antibodies in rabbits.
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Nakazawa, M., Mukumoto, M., Miyatake, K. (2010). Production and Purification of Polyclonal Antibodies. In: Schwartzbach, S., Osafune, T. (eds) Immunoelectron Microscopy. Methods in Molecular Biology, vol 657. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-60761-783-9_5
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DOI: https://doi.org/10.1007/978-1-60761-783-9_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-60761-782-2
Online ISBN: 978-1-60761-783-9
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